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CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I

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CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I

Model Number : In-Hu3427

Place of Origin : CHINA

MOQ : 1 Box

Price : Negotiable

Payment Terms : L/C, Western Union, T/T

Supply Ability : 1000kits/week

Delivery Time : 5-8 working days

Packaging Details : 96wells/box or 48wells/box

Package Size : 48tests/Kit & 96 tests/Kit

Purpose : For research use only

Specification : 48wells/96wells

Method : Sandwich

Cat. No. : In-Hu3427

Storage : 2-8℃

Brand Name : BIOVANTION

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Human Chromogranin A,CgA/Pituitary secretory protein I ELISA Kit

Human Chromogranin,CgA ELISA Kit

For research use only.
Our Human Chromogranin A (CHGA) ELISA kit is to assay Chromogranin A (CHGA) levels in Human serum, plasma, culture media or any biological fluid.
Expiration date:six months .

l Storage: 2-8℃.

Package Size :96 tests/Kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Chromogranin A (CHGA) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Chromogranin A (CHGA) and HRP conjugated Chromogranin A (CHGA) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Chromogranin A (CHGA). You can calculate the concentration of Chromogranin A (CHGA) in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

	Materials provided with the kit	48 determinations	96 determinations	Storage
1	User manual	1	1	R.T.
2	Closure plate membrane	2	2	R.T.
3	Sealed bags	1	1	R.T.
4	Microelisa stripplate	1	1	2-8℃
5	Standard:540pg/ml	0.5ml×1 bottle	0.5ml×1 bottle	2-8℃
6	Standard diluent	1.5ml×1 bottle	1.5ml×1 bottle	2-8℃
7	HRP-Conjugate reagent	3ml×1 bottle	6ml×1 bottle	2-8℃
8	Sample diluent	3ml×1 bottle	6ml×1 bottle	2-8℃
9	Chromogen Solution A	3ml×1 bottle	6ml×1 bottle	2-8℃
10	Chromogen Solution B	3ml×1 bottle	6ml×1 bottle	2-8℃
11	Stop Solution	3ml×1 bottle	6ml×1 bottle	2-8℃
12	Wash solution	20ml (20X)×1bottle	20ml (30X)×1bottle	2-8℃

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HumanChromogranin A (CHGA) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human Chromogranin A (CHGA) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I

Assay range

6pg/ml -400pg/ml

Sensitivity:

1.2pg/ml

Product Tags:

CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I Images

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CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I

CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I

CgA ELISA RUO Test Kit

human chromogranin a elisa kit

Human CgA RUO Test Kit

CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I Images